Fig. 6.

Vps33b and Vipar deficient murine epidermis shows defects in LB function. A – TEM images of the SG-SC junction in control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 epidermis. Bubble-like inclusion (single arrow), abnormal membrane structures (double arrow). Scale bars = 200 nm. Images are representative of at least two independent murine biopsies per genotype. B - TEM images of SG cells in control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 epidermis. Scale bars = 200 nm. Images are representative of at least two independent murine biopsies per genotype. C - KLK5 staining in control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 skin sections, counterstained with DAPI. All KLK5 images were taken with the same microscope settings, the brightness was boosted for the KLK5 DAPI merge to illustrate the localisation of KLK5 staining. Scale bars = 20 μm. Images are a maximum projection of a z-stack and are representative of results from at least six control and three Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 independent murine biopsies. D - ORO staining of control, Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 skin sections, counterstained with DAPI. Scale bars = 20 μm. Images are a maximum projection of a z-stack and are representative of results from at least six control and three Vps33b^fl/flER^T2 and Vipas39^fl/flER^T2 independent murine biopsies. SB, SG and SC layers are indicated.