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. 2018 May;188(5):1183–1194. doi: 10.1016/j.ajpath.2018.01.011

Figure 4.

Figure 4

Bacterial indole production induces IL-10R1. A: Indole production test of cultures from E. coli K12 WT, tnaA, and tnaB mutants; plus sign indicates presence of indole. B: EC-HPLC chromatogram of supernatants collected from E. coli K12 WT and E. coli ΔtnaA. C: T84 cells were treated for 24 hours with serially diluted supernatants from E. coli WT or E. coli ΔtnaA cultures grown to stationary phase, followed by media replenishment. Germ-free mice were colonized with E. coli WT or E. coli ΔtnaA for 2 weeks followed by euthanasia. D and E: Indole concentrations in cecal contents were validated by HPLC in vehicle and colonized mice (D), and RNA was extracted from colons for real-time quantitative PCR analysis of Il10r1 expression (E). Data are expressed as means ± SEM (D and E). n = 5 (D and E, PBS and E. coli ΔtnaA); n = 4 (D and E, E. coli WT). ∗P < 0.05, ∗∗P < 0.01, t-test. + ctrl, interferon-γ at 10 ng/mL; M, molecular marker; PBS, phosphate-buffered saline; sup, supernatant; tx, treatment.