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. 2018 May;188(5):1246–1262. doi: 10.1016/j.ajpath.2018.01.008

Figure 4.

Figure 4

Total and perfused vessels are similar in the ischemic muscle of C57 and B/c mice after 6 hours of acute hind limb ischemia. A: Representative immunofluorescence images of CD31+ (platelet endothelial cell adhesion molecule 1), indicating total vessels; and systemically injected Dylight594–conjugated Griffonia simplicolia isolectin-B4 (GS-IB4) lectin, indicating perfused vessels in transverse sections of gastrocnemius muscle from both strains after 6 hours of acute hind limb ischemia. Representative images are 2× digital zoom from images. B and C: Mean total vessel CD31+ area per image in the control right [Ctrl. (R)] and ischemic left [Isch. (L)] limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. D and E: Mean total vessel (lectin-Dylight594+) area per image in the control right and ischemic left limbs represented by absolute values and the ratio of the Isch./Ctrl. limbs. Bars indicate median, interquartile range, and maximum/minimum values (C and E). Data are expressed as means ± SEM (B and D). n = 7 animals per group. ∗P < 0.05. Scale bars = 50 μm (B and D). Original magnification: ×20 (A–E).