Figure 7.

Dramatic ischemic muscle injury is not observed in additional inbred mouse strains tested. A: Representative laser Doppler perfusion images (LDPIs) of the ischemic left [Isch. (L)] and control right [Ctrl. (R)] limbs in the prone and supine positions at baseline and after 6 hours of hind limb ischemia (HLI). B: LDPI flux ratio before HLI (Pre), immediately after HLI (Post), and after 6 hours of HLI measured in the plantar paw. C: LDPI flux ratio Pre, Post, and after 6 hours of HLI measured in the medial thigh. D: Laser Doppler flowmetry (LDF) measurements made directly over the lateral head of the gastrocnemius muscles in the absence of interference from skin pigmentation after 6 hours of HLI in the Ctrl. (R) and Isch. (L) limbs. E: Percentage oxygen saturation values measured directly over the lateral head of the gastrocnemius in the Ctrl. (R) and Isch. (L) limbs. F: Representative images of hematoxylin and eosin–stained sections from the Ctrl. (R) and Isch. (L) gastrocnemius muscles. G: Representative images of Evans Blue Dye (EBD)–positive myofibers and dystrophin immunostaining in transverse sections of Isch. (L) and Ctrl. (R) gastrocnemius muscles from both strains after 6 hours of HLI. H: Peak specific force production after maximal stimulation (120 Hz) in isolated extensor digitorum longus (EDL) muscles represented in units of N/cm². I: Mean number of EBD⁺, dystrophin-positive (Dyst.⁺), or total fibers from two images for each animal assessed in the Isch. (L) gastrocnemius muscles of both strains. Data are expressed as means ± SEM (B-E, H, and I). Representative images are 2× digital zoom from images collected at ×20 (F and G). n = 4 per group (B–E); n = 3 per group (H); n = 8 BALB/c (I); n = 6 C57BL/6 (I). ^∗P < 0.05. Scale bars = 50 μm (F and G). Original magnification: ×20 (F and G); ×10 (I). AU, arbitrary unit.