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Sucrose density gradient isoelectric focusing (IEF) of flagellar extracts. After sucrose density gradient IEF and fractionation of 2 mL, the pH and the absorbance at 580 nm of each fraction were measured. Most proteins were recovered in acidic pH fractions, and the pH of fractions 13, 14 and 15 was from pH 4.5 to pH 6. (▴), pH; (◆), absorbance.
