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. 2004 Aug 10;3(3):133–139. doi: 10.1111/j.1447-0578.2004.00061.x

Figure 4.

Figure 4

Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis of the 66‐Da protein. (a) Chromatogram of the 66 kDa protein digested by trypsin. Many peaks originated from the 66 kDa protein and peaks of self‐digested peptides of trypsin were detected. Twenty‐one peaks of those originated from the 66 kDa protein. Numbers on spectrogram indicate peaks originated from the 66 kDa protein, respectively. (b) Molecular mass and amino acid sequences of each peak originated from the 66 kDa protein. All estimated amino acid sequences were identical to the tubulin β chain from the results of data base search. (c) Amino acid sequences of human tubulin β chain. Red letters indicate sequences covered by the results of LC‐MS/MS analysis.