In the presence of 300 mmol/L ammonia (NH3) containing medium (mTALP), the effect of individual amino acids (Gln, glutamine; Ala, alanine; Gly, glycine) at 1.0 mmol and their combinations (1.0 mmol + 1.0 mmol) and each dipeptide (AlaGln, l‐alanyl‐l‐glutamine; GlyGln, l‐glycyl‐l‐glutamine) at 2.0 mmol and their combinations (1.0 mmol + 1.0 mmol) on the percentage of (a) incorporation and (b) oxidation of 14C(U)‐glucose and on the (c) accumulation of ammonia in porcine spermatozoa over a 2–4 h incubation. Each line with an error bar represents the mean ± standard error (n = 5). Different letters indicate significant differences (P < 0.05) among the treatments in the same incubation period.