Table 3.
Effect of glutathione sham‐injection on the rate of embryos on various stage of development
| Concentration (mM) | No. of embryos (%) | |||||
|---|---|---|---|---|---|---|
| n | 2‐cell | 4‐cell | 8‐cell | Morula | Blastocyst | |
| Control | 25 | 25 (100.0) | 15 (60.0) b | 9 (36.0) b | 5 (20.0) b | 5 (20.0) b |
| GSSG | ||||||
| 0.01 | 30 | 30 (100.0) | 28 (93.3) a | 21 (70.0) a | 19 (63.3) a | 11 (36.7) a,b |
| 0.02 | 25 | 25 (100.0) | 19 (76.0) a,b | 15 (60.0) a,b | 11 (44.0) a,b | 9 (36.0) a,b |
| 0.05 | 30 | 30 (100.0) | 25 (83.3) a | 15 (50.0) a,b | 12 (33.3) a,b | 8 (26.7) a,b |
| GSH | ||||||
| 0.01 | 30 | 30 (100.0) | 28 (93.3) a | 20 (66.7) a | 17 (56.7) a | 14 (46.7) a |
| 0.02 | 20 | 20 (100.0) | 16 (80.0) a,b | 14 (70.0) a | 10 (50.0) a | 8 (40.0) a,b |
| 0.05 | 28 | 28 (100.0) | 23 (82.1) a | 19 (67.9) a | 14 (50.0) a | 10 (35.7) a,b |
Values in parentheses indicate percentage. Data are expressed as of four replicates. Each embryo contains 0.35, 0.7 and 1.75 pg glutathione for 0.01, 0.02 and 0.05 mM treatment, respectively
a,bValues with different superscripts within the same column are significantly different (P < 0.05)