Figure 6.

Effects of various mammalian PLA₂ inhibitors on the purified PLA₂ activity. The purified plant PLA₂ (pPLA₂) obtained from the Superose 12 gel filtration FPLC was pre-incubated with the indicated concentrations of mammalian PLA₂ inhibitors, ETYA (A), AACOCF₃ (B), or DTT (C), at 37°C for 10 min followed by assaying the PLA₂ activity as described in “Materials and Methods.” The inhibitors-free activity of pPLA₂, group IV cPLA₂, and sPLA₂ were 3,250, 3,010, and 3,670 cpm, respectively, under the assay condition. Ethanol (2.5%, v/v) and 5% (w/v) dimethyl sulfoxide as vehicles did not affect the PLA₂ activity. Each data point represents the means ± se of three independent experiments.