Table I.

Summary of purification of the 48-kD membrane-associated PLA₂ from leaves of broad bean

Step	Protein	Total Activity	Specific Activity	Purification	Yield
	mg	pmol min−1	pmol min−1 mg−1	fold	%
Lysate	4,770	14,310	3	1	100
SDC extracts	185	8,880	48	16	62.1
(NH4)2SO4 fractionation	98	8,134	83	28	56.8
Phenyl-5PW	2.70	1,323	490	163	9.2
DEAE-5PW	0.48	1,238	2,580	860	8.7
G3000-PW	0.31	1,190	3,840	1,280	8.3
Mono Q	0.13	750	5,770	1,923	5.2
Superose 12	0.10	690	6,900	2,300	4.8

Sequential steps used in purification of the plant PLA₂, resulting in a 2,300-fold purification with 4.8% recovery.