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Boxplots of Tpref (°C) by acclimation and experimental periods: Tpref for 0, 1, 12 or 24 h of acclimation followed by a 24 h experimental period (A); Tpref for 12 h (B) or no (C) acclimation followed by 12, 24 or 36 h experimental periods and Tpref for 12 h experimental periods, including 12 h daytime (D). Significant differences for post hoc tests are indicated by a (Adj. P < 0.005) and b (Adj. P > 0.01).
