. 2009 Nov 28;9(2):73–81. doi: 10.1007/s12522-009-0041-x

Table 2.

Effect of sodium selenite, seleon l‐methionine and/or vitamin E on progressive motility, viability in porcine spermatozoa and ammonia concentrations in the culture medium

Treatment	Progressive motility (%)		Viability (%)		Ammonia concentration (mM)
Treatment	1 h	3 h	1 h	3 h	1 h	3 h
Control (without NH₃)	50.5 ± 1.4^ab	33.2 ± 1.4^a	63.6 ± 0.5^abc	53.6 ± 5.2^a	0.15 ± 0.005^a	0.17 ± 0.005^a
NH₃	36.5 ± 1.0^c	26.0 ± 1.4^a	51.8 ± 0.5^c	39.0 ± 5.2^b	0.36 ± 0.008^b	0.39 ± 0.01^d
NH₃ + SS	45.5 ± 1.4^bc	27.8 ± 1.5^a	63.6 ± 0.5^abc	45.4 ± 5.2^ab	0.31 ± 0.005^c	0.34 ± 0.01^c
NH₃ + SeMet	63.5 ± 1.4^a	32.4 ± 0.6^a	67.2 ± 0.5^ab	53.3 ± 4.9^a	0.24 ± 0.001^d	0.24 ± 0.01^b
NH₃ + Vit‐E	40.7 ± 1.4^bc	34.7 ± 2.2^a	55.4 ± 5.2^bc	41.5 ± 5.5^ab	0.28 ± 0.01^cd	0.30 ± 0.02^bc
NH₃ + SS + Vit‐E	51.0 ± 2.8^ab	39.7 ± 1.4^ab	65.4 ± 5.2^abc	43.6 ± 5.2^ab	0.29 ± 0.01^cd	0.29 ± 0.01^bc
NH₃ + SeMet + Vit‐E	61.7 ± 5.0^a	47.2 ± 0.2^b	71.8 ± 0.5^a	49.0 ± 5.2^ab	0.26 ± 0.01^cd	0.24 ± 0.01^b
NH₃ + SS + SeMet + Vit‐E	42.5 ± 1.4^bc	32.3 ± 2.0^a	56.3 ± 5.3^abc	43.6 ± 5.2^ab	0.35 ± 0.01^b	0.38 ± 0.01^cd

Values with different letters (a–e) within the same column with different superscripts are significantly different (P < 0.05). Spermatozoa were incubated 1 and 3 h in the presence of 300 μM ammonia (NH₃) in medium (Sp‐TALP) by supplementing with sodium selenite (SS; 5μg/l), seleon l‐methionine (SeMet; 5 μg/l), vitamin E (Vit‐E; 1.0 mM) and SS + SeMet + Vit‐E (0.25 + 0.25 μg/l + 1.0 mM). All values percentages are expressed as mean ± standard error (n = 5)