. 2009 Nov 28;9(2):73–81. doi: 10.1007/s12522-009-0041-x

Table 3.

Effect of sodium selenite, seleon l‐methionine and/or vitamin E on F, B and AR pattern in the chlorteracyline (CTC) staining of porcine spermatozoa

Treatment	F pattern (%)			B pattern (%)			AR pattern (%)
Treatment	0 h	1 h	3 h	0 h	1 h	3 h	0 h	1 h	3 h
Control (without NH₃)	78.0 ± 4.6^a	60.3 ± 1.3^a	30.0 ± 2.8^ab	14.0 ± 1.7	24.1 ± 1.2^a	34.1 ± 1.7^a	8.1 ± 2.6	15.4 ± 2.6^a	36.2 ± 1.4^a
NH₃	83.0 ± 1.1^b	76.0 ± 1.1^b	46.0 ± 1.1^c	10.0 ± 1.1	15.0 ± 1.1^b	25.0 ± 1.1^b	7.2 ± 2.6	9.9 ± 0.2^b	29.9 ± 0.8^b
NH₃ + SS	81.0 ± 3.3^ab	62.3 ± 1.8^a	30.9 ± 1.5^ab	11.0 ± 1.7	22.0 ± 0.6^a	36.1 ± 1.2^a	8.9 ± 2.6	16.3 ± 0.2^a	32.7 ± 0.3^a
NH₃ + SeMet	79.6 ± 2.1^ab	57.7 ± 1.5^ac	24.0 ± 1.1^bd	10.0 ± 1.1	24.0 ± 1.3^a	39.0 ± 1.7^a	10.8 ± 2.6	18.7 ± 0.8^a	36.9 ± 0.1^a
NH₃ + Vit‐E	81.3 ± 1.9^ab	62.0 ± 2.1^a	36.1 ± 2.3^a	11.0 ± 1.2	23.5 ± 0.8^a	33.0 ± 1.7^ab	8.1 ± 2.6	15.4 ± 0.8^a	30.6 ± 1.1^ab
NH₃ + SS + Vit‐E	80.6 ± 1.5^ab	60.3 ± 3.9^a	34.0 ± 1.7^a	11.0 ± 1.7	24.0 ± 1.7^a	34.6 ± 1.4^a	8.6 ± 2.3	16.0 ± 0.7^a	31.8 ± 0.8^a
NH₃ + SeMet + Vit‐E	74.0 ± 1.2^a	51.3 ± 1.7^c	23.1 ± 1.2^d	13.0 ± 1.2	28.0 ± 1.1^a	42.0 ± 1.2^a	12.9 ± 0.8	20.9 ± 0.3^a	35.5 ± 0.3^a
NH₃ + SS + SeMet + Vit‐E	81.3 ± 1.3^ab	63.3 ± 1.3^a	32.0 ± 1.7^a	10.2 ± 0.5	22.0 ± 1.2^a	36.0 ± 1.1^a	9.3 ± 2.6	15.5 ± 2.6^a	31.8 ± 2.6^a

Values with different letters (a–d) within same the column with different superscripts are significantly different (P < 0.05). Spermatozoa were incubated 0, 1 and 3 h in the presence of 300 μM ammonia (NH₃) in medium (Sp‐TALP) by supplementing with sodium selenite (SS; 5 μg/l), seleon l‐methionine (SeMet; 5 μg/l), vitamin E (Vit‐E; 1.0 mM) and SS + SeMet + Vit‐E (0.25 + 0.25 μg/l + 1.0 mM). All values percentages are expressed as mean ± standard error (n = 5)