Table 4.
Effect of sodium selenite, seleon l‐methionine and/or vitamin E on incorporation of 14C(U)‐glucose in porcine spermatozoa
| Treatment | Incorporation of cmp/0.5 × 106 spermatozoa | |
|---|---|---|
| 1 h | 3 h | |
| Control (without NH3) | 3021.2 ± 287.85ab | 3735.3 ± 288.0a |
| NH3 | 2508.6 ± 33.6a | 3368.4 ± 150.1ae |
| NH3 + SS | 2983.8 ± 60.4ab | 3806.7 ± 193.5ab |
| NH3 + SeMet | 3325.8 ± 214.7ab | 5056.7 ± 46.1c |
| NH3 + Vit‐E | 2861.5 ± 136.2ab | 3748.7 ± 85.9a |
| NH3 + SS + Vit‐E | 3102.0 ± 130.9ab | 4492.7 ± 35.0b |
| NH3 + SeMet + Vit‐E | 3626.7 ± 307.7b | 6859.0 ± 215.6d |
| NH3 + SS + SeMet + Vit‐E | 2735.2 ± 99.1a | 2742.5 ± 133.1e |
Values with different letters (a–f) within the same column with different superscripts are significantly different (P < 0.05). Spermatozoa were incubated 1 and 3 h in the presence of 300 μM ammonia (NH3) in medium (Sp‐TALP); by supplementing with sodium selenite (SS; 5 μg/l), seleon l‐methionine (SeMet; 5 μg/l), vitamin E (Vit‐E; 1.0 mM) and SS + SeMet + Vit‐E (0.25 + 0.25 μg/l + 1.0 mM). All value percentages are expressed as mean ± standard error (n = 5)