Effects of 17β‐estradiol (E) on progesterone (P)‐enhanced hyperactivation when hamster spermatozoa were exposed to P after exposure to E for 5 min. Each experimental condition is shown in Table 1. It shows an overview of the effects of P and E on the percentage of hyperactivation (a, c, e) and details of significant effects (b, d, f). Data are expressed as mean ± SD. In all graphs, “Vehicle” refers to mTALP medium + 0.2 % ethanol. In a and b, “10 ng/mL P” is vehicle + 10 ng/mL P. P + all concentrations of E are vehicle + 10 ng/mL P + respective concentration of E. In c and d, “20 ng/mL P” is vehicle + 20 ng/mL P. P + all concentrations of E are vehicle + 20 ng/mL P + respective concentration of E. In e and f, “40 ng/mL P” is vehicle + 40 ng/mL P. P + all concentrations of E are vehicle + 40 ng/mL P + respective concentration of E. *Significant difference compared with “Vehicle” (P < 0.05). **Significant difference compared with “10 ng/mL P”, “20 ng/mL P” or “40 ng/mL P” (P < 0.05). ***Significant difference compared with “Vehicle” and “40 ng/mL P” (P < 0.05)