Figure 5.

Protein serine phosphorylation during hyperactivation. (a) Western blotting of the urea extracts. (b) Western blotting of the urea‐thiourea extracts. Lanes a–e and f–j showed Coomassie Brilliant Blue stain of membrane and enhanced chemiluminescence, respectively. Lanes a and f, lanes b and g, lanes c and h, lane d and i, and lane e and j showed 0 min, 30 min, 60 min, 120 min and 180 min, respectively. Arrows show protein phosphorylations detected as a significant reaction on statistical analysis by one‐factor anova. Eight phosphoproteins detected on (a) were designated as pS90u, pS75u, pS50u, pS40u, pS33u, pS32u, pS30u and pS23u. Six phosphoproteins detected on (b) were designated as pS82ut, pS52ut, pS45ut, pS40ut, pS34ut and pS23ut. Numbers of left side show molecular markers. Experiment number was five. (c) Relative density of significant phosphorylations detected on (a) and (b). When reactivities of 0 min were taken, reactivities of each incubation time were showed.