Figure 7.

Protein phosphorylation by A‐kinase during hyperactivation. (a) Western blotting of the urea extracts. (b) Western blotting of the urea‐thiourea extracts. Lanes a–e and f–j showed Coomassie Brilliant Blue stain of membrane and enhanced chemiluminescence, respectively. Lanes a and f, lanes b and g, lanes c and h, lanes d and i, and lanes e and j showed 0 min, 30 min, 60 min, 120 min and 180 min, respectively. Arrows showed protein phosphorylations detected as a significant reaction on statistical analysis by one‐factor anova. Numbers of left side show molecular markers. Experiment number was five. (c) Relative density of significant phosphorylations detected on (a) and (b). When reactivities of 0 min were taken, reactivities of each incubation times are shown.