Figure 4.

Comparison of ARS reductase protein antigenicity from marine algae. All of the lanes were loaded with equal amount of protein (6 μg) except for Ulva pertusa (30 μg) and the purified recombinant protein from pET-EAPR (2 ng). Odd-numbered lanes are controls blotted with preimmune antiserum, whereas even-numbered lanes are blotted with APR antiserum. A, EI (E. intestinalis): total activity was 120 pmol min⁻¹; UP (U. pertusa): total activity was 16 pmol min⁻¹; Tetra (Tetraselmis sp.): total activity was 1,300 pmol min⁻¹; Dun (D. salina): total activity was 390 pmol min⁻¹; Pure APR (purified recombinant protein from pET-EAPR): total activity was 90 pmol min⁻¹. B, Tetra (Tetraselmis sp.): total activity was 1,300 pmol min⁻¹; Iso (I. galbana): total activity was 1,480 pmol min⁻¹; Ehux (E. huxleyi): total activity was 1,500 pmol min⁻¹; TW (T. weissflogii): total activity was 1,400 pmol min⁻¹; TO (T. oceanica): total activity was 800 pmol min⁻¹; HT (H. triquetra): total activity was 30 pmol min⁻¹.