Fig. 5.

Intra-amniotic exposure to LPS induces distinct time-dependent changes in oligodendrocyte lineage cells. A significant decrease of Olig2-positive cells was observed in animals after 15 days of LPS exposure compared to controls (SAL vs. 15 days LPS p = 0.050) (c). At the same time point, a decrease in PDGFRa-positive cells was found compared to controls (SAL vs. 15 days LPS p = 0.070) (d). No significant changes of CNPase+ cells were found following LPS exposure compared to controls (e). Area fractions (%) of MBP immunoreactivity (IR) showed a decrease at 2 days and an increase at 8 days following LPS exposure compared to controls (SAL vs. 2 days LPS, p = 0.070; SAL vs. 8 days LPS p = 0.083) (f). Representative histological figures of Olig2, PDGFRa, and CNPase-positive cells and MBP IR in animals exposed to intra-amniotic saline (SAL) and 15 days of LPS are shown in a and b respectively. Images taken at × 100 magnification (insert at × 400 magnification), scale bar = 200 μm. Asterisk indicated p < 0.05 versus control (SAL); number sign indicated 0.05 < p < 0.1 versus control (SAL)