Table I.
Incorporation of deuterium from l-[2H5]Trp into IAA and Trp
| Compound | Ion | A | B | C |
|---|---|---|---|---|
| % | ||||
| Trp | 202 | 36 | 53 | 53 |
| 207 | 64 | 47 | 47 | |
| IAA | 130 | 11 | 76 | 61 |
| 134 | 42 | 21 | 29 | |
| 135 | 47 | 3 | 10 | |
| 134 + 135 | 89 | 24 | 39 | |
| 189 | 11 | 75 | 62 | |
| 193 | 41 | 20 | 23 | |
| 194 | 48 | 5 | 15 | |
| 193 + 194 | 89 | 25 | 38 | |
IAA and tryptophan isolated from kernels labeled with 20 mm l-[2H5]Trp (A), 2 mm l-[2H5]Trp (B), 2 mm l-[2H5]Trp, and 2 mm unlabeled indole as competitor (C) were analyzed by GC-MS. For Trp the relative mass distribution of the N-sylilated quinolinium ion is shown (m202 + m207 = 100%). For IAA the relative mass distribution of the quinolinium ion (m189 + m193 + m194 = 100%) and the molecular ion (m189 + m193 + m194 = 100%) is shown. The identity of [ring-1H5]IAA (m = 130), [ring-2H4]IAA (m = 134), and [ring-2H5]IAA (m = 135) was confirmed by HR EI-MS.