Fig. 4.

Identification of urethral carcinoma-associated 1 (UCA1)-associated micro RNAs (miRNAs). a MS2 pulldown was performed in MDA231/UCA1-MS2 cells. Enrichment of UCA1-MS2 in the precipitates was measured by RT-qPCR: **P < 0.01. b After MS2 pulldown, the levels of selected miRNAs associated with UCA1 were measured by RT-qPCR. The data are presented as means ± SD from three independent experiments: **P < 0.01 as determined by Student’s t test. c Shows the putative binding sites for insulin-like growth factor 2 messenger RNA binding protein (IMP1) and miR-122-5p. The mutated miR-122-5p binding site on UCA1 is shown as green. d At 24 h after transfection with either UCA1-MS2 or UCA1m-MS2 construct, 293 T cells were lysed and the lysates were analyzed by pulldown assays using MBP-MCP-conjugated amylose resin. Both MS2-tagged UCA1 and UCA1m RNAs were precipitated. e Interaction of miR-122-5p with UCA1-MS2 was examined in the pulldown material. Relative levels of miR-122-5p in the precipitates are represented as mean ± SD from three independent experiments: *P < 0.05, **P < 0.01