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. Author manuscript; available in PMC: 2018 Apr 19.
Published in final edited form as: Cancer Res. 2012 Nov 1;73(1):331–340. doi: 10.1158/0008-5472.CAN-12-3151

Figure 5. AXL blocks binding of c-ABL to p73β protein and attenuates CDDP-induced phosphorylation of c-ABL.

Figure 5

A) OE33 cells stably expressing AXL or pcDNA4 were treated with vehicle or CDDP (10 μmol/L) for 48h. The Western blot data indicate that CDDP treatment significantly increased p-c-ABL(Y412), p-p73β(Y99), and p73β protein levels in control cells. In contrast, AXL expression abrogated these effects in response to CDDP. B) Western blot analysis of immunoprecipitated exogenous p73β protein with M2-flag antibody in OE33 cells transfected with p73β-Flag alone or in combination with AXL. Exogenous p73β interacted with endogenous c-ABL protein, and the p73β/c-ABL protein complex was disrupted by AXL. C) Cell viability of OE33 cells transfected with pcDNA4 alone, AXL alone or in combination with GFP-c-ABL was assessed 48h post-transfection. AXL expression blocked c-ABL-induced cell death. D) Western blot analysis of c-ABL and AXL proteins in OE33 cells transiently transfected as in panel C.