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. Author manuscript; available in PMC: 2018 Apr 19.
Published in final edited form as: Cancer Res. 2012 Nov 1;73(1):331–340. doi: 10.1158/0008-5472.CAN-12-3151

Figure 6. AXL interacts with c-ABL protein and attenuates CDDP-induced accumulation of c-ABL in the nucleus.

Figure 6

A) Western blot analysis of immunoprecipitated proteins with AXL antibody in OE33 cells stably expressing pcDNA4 or AXL. The data demonstrate protein association of AXL with endogenous c-ABL. B) Western blot analysis of immunoprecipitated proteins with AXL antibody in HEK-293 cells transiently co-transfected with AXL and GFP-c-ABL. The results indicate protein interaction between AXL and exogenous c-ABL. C) Western blot analysis of cytosolic and nuclear fractions of OE33 cells stably expressing pcDNA4 or AXL and treated with vehicle or CDDP (10 μmol/L) for 48h. The data show accumulation of c-ABL in the nucleus in control cells whereas c-ABL was mainly sequestered in the cytosol and undetected in the nucleus in AXL-expressing cells in response to CDDP.