Skip to main content
. 2018 Mar 7;7(6):e007572. doi: 10.1161/JAHA.117.007572

Figure 2.

Figure 2

miR‐1281 suppresses pulmonary artery smooth muscle cell (PASMC) proliferation and migration. A, Real‐time quantitative reverse transcription–polymerase chain reaction (qRT‐PCR) analysis of overexpression level of miR‐1281 in PASMCs achieved by transfection of miR‐1281 mimic. B, Representative figures of EdU assay and statistical analysis showing that miR‐1281 mimic inhibited PASMC proliferation under culturing conditions without and with platelet‐derived growth factor (PDGF) BB treatments. C, qRT‐PCR analysis of inhibitory efficiency of miR‐1281 inhibitor in PASMCs. D, Representative figures of EdU assay and statistical analysis showing that miR‐1281 inhibitor promoted PASMC proliferation. E, Representative figures of wound‐healing assay and statistical analysis showing that miR‐1281 mimic inhibited PASMC migration under culturing conditions without and with PDGFBB treatments. F, Representative figures of wound‐healing assay and statistical analysis showing that miR‐1281 inhibitor promoted PASMC migration. DAPI indicates 4′,6‐diamidino‐2‐phenylindole; and NC, negative control. *P<0.05, **P<0.01, ***P<0.001 compared with control or as specified by broken lines in respective figures. Exact P values in consecutive order are: 0.00045, 0.012642, 0.001155, 0.001081, 0.009504,0.03544, 0.003835, 0.002613.