Figure 7.
LPS reduces plasma CETP concentration that correlates with hepatic VSIG4 expression in humans. Ninety‐three liver biopsies were taken during bariatric surgery, and assayed for gene expression by microarray analysis. The correlation between the expression of CETP and VSIG4 (A) and CD14 (B), and plasma CETP concentration and VSIG4 expression (C) were determined, and the goodness of fit R 2 from linear regression analyses was shown. D, Twenty healthy males received a bolus infusion of 1 ng/kg body weight of LPS via the antecubital vein. After an overnight fast, blood samples were collected before (baseline) and 24 hours after LPS infusion, and plasma CETP concentration was determined. ***P<0.001 as compared with the baseline. E through G, Human monocyte‐derived macrophages were treated with vehicle (control group), LPS (LPS group), TO‐901317 (LXR group) and LPS+TO‐901317 (LPS+LXR group). The mRNA expression of ABCG1,CETP and VSIG4 was normalized to the Control group. Data are presented as means±SEM (n=4). *P<0.05, **P<0.01, ***P<0.001. ABCG1 indicates ATP binding cassette subfamily G member 1; CD14, cluster of differentiation 14; CETP, cholesteryl ester transfer protein; LPS, lipopolysaccharide; VSIG4, V‐set and immunoglobulin domain containing 4.