Figure 7.

Effects of IFN‐γ on the activation of Stat5 and Akt in in vitro cultured neonatal rat cardiomyocytes. A, Western blotting analyses were conducted to detect Stat5 and Akt in neonatal rat cardiomyocytes with or without mechanical stretch. Representative results from 5 independent experiments are shown here. Relative amounts of p‐Stat5 (B) and p‐Akt (C) to GAPDH were analyzed by densitometry and calculated. All values represent means±SEM (n=5). *P<0.05; **P<0.01. D through F, Effects of the Stat5 inhibitor on the IFN‐γ‐induced phosphorylation of Stat5 and Akt in rat cardiomyocytes, which were cultured under the stretch condition. Relative amounts of p‐Stat5 (E) and p‐Akt (F) to GAPDH were analyzed by densitometry and calculated. All values represent means±SEM (n=5). *P<0.05; **P<0.01. Akt indicates protein kinase B; GAPDH, glyceraldehyde 3‐phosphate dehydrogenase; IFN‐γ, interferon‐γ; Stat5, signal transducer and activator of transcription 5.