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Substrate dependence of rubber transferase activity in fig tree WRP. Five milligrams of WRP was incubated with increasing amounts of FPP at saturating IPP concentration (1 mm; A), and increasing amounts of IPP at saturating FPP concentration (20 μm; B) at 25°C for 5 h. Product formation was plotted against substrate concentration, and the apparent Km values were calculated by a Lineweaver-Burk analysis.
