Fig. 2.

Reconstructed ion images of selected lipid species found via MALDI–MSI and confirmed by LC–MS on barley root sections under control (left panels) and 150 mM NaCl (centre panels) conditions. Right panels show a bar graph of the relative response of the lipid found on different root zones (Z1—root cap and cell division zone; Z2—zone of elongation; Z3 - zone of maturation) under control (C) and salt (S). Images were recorded with a scanning step size of 30 × 30 μm. The MS images are of PC (34:3) (m/z 756.5496), PC (38:9) (m/z 800.5252), PA (36:6) (m/z 715.4262), SQDG (28:2) (m/z 735.4326), MAG (34:5) (m/z 573.4873), and LPC (20:5) (m/z 542.3223). Scale bars: 500 μm. Control and salt treated images have been set to the same intensity scale and obtained from the same MALDI–MSI experiment