Table 1.
Apoptosis (%) |
|||
---|---|---|---|
Mean | SD | p-value | |
Medium | 23 | 21 | |
AaPAL 0.5 µg/mL | 32 | 37 | 0.471 |
AaPAL 2.0 µg/mL | 56 | 33 | 0.045 |
LP 1.0 µg/mL | 30 | 24 | 0.044 |
LPS 2.0 µg/mL | 20 | 19 | 0.628 |
Cells were stimulated with different concentrations of AaPAL for 48 h. The apoptosis was estimated by measuring DNA fragmentation using the TiterTACS kit (R&D systems). LP denotes synthetic bacterial lipopeptide Pam3CSK4 (EMC Microcollections GmbH). Nuclease-treated cells were used as a positive control and regarded as 100% apoptotic cells. The table shows means and SD from three separate experiments. The statistical significance of the difference between the treated and non-treated (medium) cells was evaluated using the Mann-Whitney U-test. p-values <0.05 were considered statistically significant.