FIG 1 .

amrZ is a positive regulator of swimming motility and colony spreading in Pseudomonas stutzeri. Strains DBL1052 and DBL1053 are derived from strains DBL332 and DBL390, respectively, and contain independently created deletions in amrZ. Individual data points for each assay are plotted for each strain, with boxes representing 2 standard deviations and means plotted as horizontal blue lines at the center of the box plots. Measurements within each assay have been normalized so that the value of DBL332 is 1. Letters above each box plot indicate that mean values are significantly different at P < 0.01 according to Tukey’s HSD test. (A) AmrZ is a positive regulator of swimming motility, and loss of swimming motility is seen in independently created amrZ deletion lines. (B) Representative images from motility assays are shown for a wild-type strain, an amrZ deletion strain, and an flgK deletion strain. In each case, colored circles have been created that match halos in these pictures and have been superimposed in the final image (blue, DBL332; gray, DBL1052; white; DBL1168). (C) AmrZ is a positive regulator of colony spreading, and loss of spreading is seen in independently created amrZ deletion lines. (D) Representative example of colony spreading activity and positive regulation by AmrZ. The same plate is shown after being scanned after 1 day of growth (left) and after 3 days of growth (right). AmrZ⁺ strains spread outward on KB medium over time, while AmrZ⁻ strains fail to spread unless there are compensatory mutations (as shown by the white arrow in the DBL1052 strain). wt, wild type.