FIG 6 .

The gshA transposon mutants (PW4072 and PW9759) are deficient in pyocyanin production. Spent medium supernatants of cultures of each strain grown overnight in TSB were extracted with chloroform and HCl, and absorbance readings were taken at 520 nm for pyocyanin quantification. There was significantly less pyocyanin production in the gshA transposon mutant strain compared to the wild-type (MPAO1), complemented (TJB10 and BH01), and control (PW5404) strains. Values are means plus standard deviations from five independent experiments. Values that are significantly different from the value for the wild-type MPAO1 strain are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01.