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. 2018 Apr 18;3(2):e00155-18. doi: 10.1128/mSphere.00155-18

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Copyright © 2018 Van Laar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 8 — Persister cell formation is defective in gshA transposon mutants (PW4072 and PW9759). Persister cells were formed by exposing one stationary-phase culture to 50 µg/ml ofloxacin for 3.5 h. The number of CFU of untreated cultures was compared to that of ofloxacin-treated cultures to calculate the percentage of persister cells formed. The two mutant strains (PW4072 and PW9759) produced few to no persister cells, while the cis-complemented strain (BH01) produced significantly more persister cells than the wild-type strain (MPAO1). Values are means plus standard deviations from four independent experiments. Values that are significantly different from the value for the wild-type MPAO1 strain are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01.