Fig. 3. : localisation of mRNA. (A) Fluorescent in situ hybridisation assay using specific oligo (dT) probe. As the control, RNase A was incubated with the parasites before probe hybridisation. DAPI: DNA stained with DAPI; mRNA: mRNA detection; granules: labelling of pixels with high intensity fluorescence signals; N: nucleus; K: kinetoplast. Scale bar = 5 µm. (B) Histogram of mRNA granule counts per cell.