Figure 2.

Quantitative polymerase chain reaction (qPCR) data. A, Raw amplification plots generated during qPCR targeting the 605-base pair green fluorescence protein DNA fragment (GFP₆₀₅) spike-in in eluents yielded from plasma DNA extraction, overlaid with those of the standard curve. AU indicates arbitrary units. B, Melting plots generated during subsequent melting point analysis. Individual lines indicate averaged polymerase chain reaction (PCR) technical replicates associated with each DNA extraction.