Figure 4.

DNA extraction efficiency. A, Crossing threshold (CT) values generated during quantitative polymerase chain reaction (qPCR) targeting the 605-base pair green fluorescence protein DNA fragment (GFP₆₀₅) spike-in in DNA extraction eluents across all specimens for each group. ANOVA indicates analysis of variance; CV, coefficient of variation. B, Corresponding DNA extraction efficiencies. Points and error bars depict averages, and SDs associated with DNA extraction technical replicates for each specimen. Boxes depict averages and SDs across biological replicates for each group.