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Tracking phenotypic PIG-A status through apoptosis and necrosis. Phenotypic PIG-A mutant frequency data for MCL-5 cells undergoing (A) apoptosis and (B) necrosis following a time course acute 1 µM staurosporine exposure: (1) 30 min, (2) 60 min, (3) 120 min, (4) 240 min and (5) 480 min. Relative PE detector signals utilised as the measure for surface GPI-AP expression (>3000 events analysed in each sample). ‘PIG-A WT’ gate present defines threshold for phenotypic PIG-A mutant status.
