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. Author manuscript; available in PMC: 2018 Sep 5.
Published in final edited form as: Biochemistry. 2017 Aug 21;56(35):4637–4645. doi: 10.1021/acs.biochem.7b00638

Figure 5.

Figure 5

Human Sts-1HP dephosphorylates Zap-70. Using a cell-based assay (see Materials and Methods) both mouse and human Sts-1HP were tested for their ability to dephosphorylate the hyperphosphorylated substrate, Zap-70. In the absence of either Sts-1 protein, a dark band is seen for phosphorylated Zap-70 (phospho-Zap-70, top lane of the gel). When either mouse Sts-1 (m) or human Sts-1 (h) are also present in the cells, the amount of phosphorylated Zap-70 is significantly diminished without an effect on the overall expression level of the Zap-70 protein. This experiment was conducted in triplicate and the blot shown is a representative example of these data.