. Author manuscript; available in PMC: 2018 Sep 5.

Published in final edited form as: Biochemistry. 2017 Aug 21;56(35):4637–4645. doi: 10.1021/acs.biochem.7b00638

Table 1.

Kinetic constants of human Sts proteins

Substrate/Enzyme	K_m (mM)	k_cat (s⁻¹)	k_cat / K_m (M⁻¹s⁻¹)
pNPP
human Sts-1_HP	2.64 ± 0.48	13.62 ± 0.63	5.16 × 10³
human Sts-2_HP	5.61 ± 1.69	1.23 ± 0.15	2.19 × 10²
Full-length Sts-1	3.31 ± 0.27	158.6 ± 6.48	4.77 × 10⁴

OMFP

human Sts-1_HP	0.19 ± 0.03	786.1 ± 52.4	4.14 × 10⁶
human Sts-2_HP	1.07 ± 0.24	5.86 ± 0.78	5.48 × 10³
Full-length Sts-1	ND	ND	ND

DifMUP

human Sts-1_HP	0.002	100.68	5.03 × 10⁷
human Sts-2_HP	0.85 ± 0.23	4.92 ± 0.76	5.79 × 10³
Full-length Sts-1	ND	ND	ND