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RNA gel-blot analysis of TBG spatial expression in fruit tissues. Twenty micrograms of total RNA extracted from mature green or turning stage fruit peel (P), outer pericarp (OP), inner pericarp (IP), and locular (L) tissue was loaded in each lane. Blots were hybridized using the probes indicated to the right. A cDNA clone for PG was used as a fruit-ripening-specific control. A 26S ribosomal gene clone from soybean was used as a loading control for each blot; one example is shown.
