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. 2018 Apr 9;14(4):e1006975. doi: 10.1371/journal.ppat.1006975

Fig 4. Janus kinases (JAK) are responsible for mediating Y705 phosphorylation in E6 and HPV18 containing keratinocytes.

Fig 4

A) Representative western blot of keratinocytes subjected to high calcium differentiation analysed with antibodies specific for the total and phosphorylated forms of JAK2 (Y1007/1008). GAPDH served as a loading control. Data shown are representative of at least three independent biological repeats. B) Representative western blot of C33a cells transfected with GFP and GFP tagged HPV18 E6 and analysed using specific antibodies detecting phosphorylated (Y1007/1008) and total JAK2. Expression of E6 was confirmed using a GFP antibody. GAPDH served as a loading control. C) Representative western blots of HPV18 containing keratinocytes incubated with the JAK1/2 inhibitor Ruxolitinib or JAK2 inhibitor Fedratinib analyzed with antibodies detecting total and phosphorylated forms of JAK2 and STAT3. GAPDH served as a loading control. Data shown are representative of at least three independent biological repeats.