Table 1. List of ONE- and HNE-modified HSA peptides identified from the reaction of HSA with 13-HPODE in the presence of AscA.
| Peptide Sequence | Modification | Peptides monitored | |
|---|---|---|---|
| Site | Type | ||
| ALVLIAFAQYLQQCPFEDH*VK | H39 | ONE, HNE | |
| SLH*TLFGDK | H67 | HNE | HH1 |
| SLH*TLFGDKLCTVATLR | H67 | HNE | |
| SLHTLFGDK*LCTVATLR | K73 | ONE, ONE−H2O | HO1 |
| LVRPEVDVMCTAFHDNEETFLK*K | K136 | ONE−H2O | |
| K*YLYEIAR | K137 | ONE−H2O | HO2 |
| R*H*PYFYAPELLFFAK | R145/H146 | ONE/HNE | |
| H*PYFYAPELLFFAK | H146 | HNE | HH2 |
| RH*PYFYAPELLFFAK | H146 | ONE, HNE | |
| RYK*AAFTECCQAADK | K162 | ONE, ONE−H2O | |
| YK*AAFTECCQAADK | K162 | ONE, ONE−H2O | HO3 |
| LDELRDEGK*ASSAK | K190 | ONE−H2O, HNE−2H2O | HO4, HH3 |
| CASLQK*FGER | K205 | ONE−H2O, HNE−2H2O | |
| AFK*AWAVAR | K212 | ONE−H2O | HO5 |
| VH*TECCHGDLLECADDR | H242 | HNE | |
| VH*TECCHGDLLECADDRADLAK | H242 | HNE | HH4 |
| VH*TECCH*GDLLECADDR | H242/H247 | HNE/ONE, HNE | |
| VH*TECCH*GDLLECADDRADLAK | H242/H247 | HNE/HNE | |
| SH*CIAEVENDEMPADLPSLAADFVESK | H288 | HNE | HH5 |
| R*H*PDYSVVLLLR | R337/H338 | ONE/HNE | |
| RH*PDYSVVLLLR | H338 | HNE | |
| VFDEFK*PLVEEPQNLIK | K378 | ONE | |
| NLGK*VGSK | K432 | ONE | HO6 |
| EFNAETFTFH*ADICTLSEK | H510 | HNE | HH6 |
| K*QTALVELVK | K525 | ONE−H2O | HO7 |
* indicates a modification site.