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. 2018 Mar 2;22(5):2656–2669. doi: 10.1111/jcmm.13544

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© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Dach1 and Synpo mRNA levels were reduced after cultivation of isolated glomeruli. A, Isolated glomeruli express CFP in podocytes under control of the nephrin promoter. BF—bright field. Scale bars represent 25 μm. B, As determined by RT‐PCR, Dach1 and Synpo mRNA expression significantly decreased after cultivation of glomeruli for 9 days (n = 3). C, RNA sequencing of isolated glomeruli from day 0, 3, 6 and 9 confirmed a significant decrease in the mRNA of Dach1 and Synpo during dedifferentiation. ****P < .0001, ***P < .001, **P < .01, *P < .05