Figure 4.

The knockdown of USP4 activates p53 signalling in melanoma cells under cisplatin treatment. A and B, The expressions of Cleaved‐caspase 9, Bcl‐2 and Bax was analysed by immunoblotting in melanoma cells with indicated treatment. The right panel is a densitometry analysis of 3 individual experiments. Cis represents cisplatin. C, The protein level of p53 was analysed by immunoblotting in melanoma cells with indicated treatment. D, The mRNA level of p53 was analysed by qRT‐PCR in melanoma cells with indicated treatment. Data are presented as the mean ± SD, *P < .05, **P < .01, ***P < .001; Student's t test