Figure 1.

Luciferase assay demonstrates that 20 μmol/L of BAY 11‐7082 prevents the NF‐κB transcriptional activity in acidic bile‐treated normal human hypopharyngeal cells (HHPC) and human hypopharyngeal keratinocytes (HHK). A, Columns of graphs represent luciferase activity (mean ± standard error of three independent experiments) in (a) HHPC and (b) HHK, transfected with control luciferase reporter (luc2P) and NF‐κB luciferase responsive element (luc2P‐NF‐κB‐RE). B, Columns of graphs represent NF‐κB relative transcriptional activity (luc2P‐NF‐κB‐RE: NF‐κB luciferase responsive element/luc2P: control luciferase reporter) with/without BAY 11‐7082, in (a) HHPC and (b) HHK. Luc, luciferase