Table 2.
List of DEmiRNAs
miRNA ID | C14MC | C19CM | Whole plasma | EV | EV‐depleted plasma | |||
---|---|---|---|---|---|---|---|---|
Fold change (log2) PTL/control | P‐value | Fold change (log2) PTL/control | P‐value | Fold change (log2) PTL/control | P‐value | |||
hsa‐miR‐100‐5p | −0.82 | 4.63E−02 | ||||||
hsa‐miR‐1‐1‐3p | −0.86 | 1.76E−02 | ||||||
hsa‐miR‐1179‐5p | −0.83 | 3.94E−06 | ||||||
hsa‐miR‐1185‐1‐3p | X | −0.82 | 3.02E−03 | |||||
hsa‐miR‐1185‐1‐5p | X | −0.95 | 1.76E−04 | |||||
hsa‐miR‐1185‐2‐3p | X | −0.72 | 2.29E−03 | |||||
hsa‐miR‐1246‐5p | 2.10 | 3.90E−07 | ||||||
hsa‐miR‐1249‐3p | −1.23 | 3.81E−04 | −1.04 | 3.46E−02 | ||||
hsa‐miR‐1256‐3p | −0.61 | 4.34E−03 | ||||||
hsa‐miR‐126‐3p | −0.63 | 1.09E−03 | ||||||
hsa‐miR‐126‐5p | −0.62 | 5.66E−04 | ||||||
hsa‐miR‐127‐3p | X | −0.97 | 1.53E−03 | |||||
hsa‐miR‐127‐5p | X | −0.75 | 1.16E−02 | −0.94 | 1.91E−02 | |||
hsa‐miR‐1277‐3p | −1.42 | 5.46E−09 | ||||||
hsa‐miR‐1277‐5p | −1.20 | 3.42E−06 | ||||||
hsa‐miR‐130a‐3p | −0.69 | 2.10E−03 | ||||||
hsa‐miR‐130a‐5p | −0.62 | 1.15E−03 | ||||||
hsa‐miR‐1343‐3p | −0.68 | 4.79E−03 | ||||||
hsa‐miR‐135a‐1‐5p | −1.06 | 7.26E−06 | ||||||
hsa‐miR‐136‐3p | X | −0.68 | 2.40E−02 | −0.83 | 1.66E−02 | |||
hsa‐miR‐136‐5p | X | −0.84 | 6.39E−03 | −1.14 | 5.38E−03 | |||
hsa‐miR‐141‐3p | −0.81 | 7.49E−03 | ||||||
hsa‐miR‐142‐3p | −0.65 | 1.94E−03 | ||||||
hsa‐miR‐146b‐5p | −0.69 | 8.22E−05 | ||||||
hsa‐miR‐153‐1‐3p | −0.61 | 5.55E−04 | ||||||
hsa‐miR‐154‐3p | X | −0.75 | 3.77E−03 | −0.92 | 4.46E−02 | |||
hsa‐miR‐154‐5p | X | −0.69 | 7.13E−03 | |||||
hsa‐miR‐155‐5p | −0.69 | 6.57E−04 | ||||||
hsa‐miR‐181b‐2‐5p | −0.61 | 4.16E−04 | ||||||
hsa‐miR‐181c‐3p | −0.66 | 3.01E−03 | ||||||
hsa‐miR‐181c‐5p | −0.96 | 1.63E−06 | ||||||
hsa‐miR‐181d‐5p | −0.77 | 1.98E−04 | ||||||
hsa‐miR‐183‐5p | 0.63 | 1.69E−03 | ||||||
hsa‐miR‐186‐3p | −0.84 | 3.06E−04 | ||||||
hsa‐miR‐192‐5p | 0.71 | 2.26E−02 | ||||||
hsa‐miR‐193a‐5p | 0.96 | 9.50E−05 | ||||||
hsa‐miR‐193b‐3p | 0.70 | 1.60E−02 | ||||||
hsa‐miR‐194‐1‐5p | 0.63 | 3.55E−02 | ||||||
hsa‐miR‐199a‐1‐5p | −0.89 | 7.18E−05 | ||||||
hsa‐miR‐19b‐2‐3p | −0.72 | 2.15E−05 | ||||||
hsa‐miR‐20a‐3p | −0.85 | 6.27E−06 | ||||||
hsa‐miR‐214‐3p | 0.69 | 4.40E−02 | ||||||
hsa‐miR‐215‐5p | −0.79 | 2.07E−02 | ||||||
hsa‐miR‐219a‐1‐5p | −0.84 | 1.37E−05 | ||||||
hsa‐miR‐221‐3p | −0.61 | 6.16E−04 | ||||||
hsa‐miR‐26a‐1‐5p | −0.76 | 1.17E−04 | ||||||
hsa‐miR‐26a‐2‐3p | −0.60 | 2.91E−04 | ||||||
hsa‐miR‐26b‐5p | −0.80 | 9.30E−06 | ||||||
hsa‐miR‐299‐3p | X | −0.64 | 2.17E−02 | |||||
hsa‐miR‐299‐5p | X | −0.81 | 2.25E−03 | −0.82 | 1.79E−02 | |||
hsa‐miR‐29a‐5p | −0.62 | 4.49E−04 | ||||||
hsa‐miR‐29b‐2‐3p | −1.11 | 2.93E−07 | ||||||
hsa‐miR‐301a‐3p | −0.87 | 3.85E−07 | ||||||
hsa‐miR‐301b‐3p | −0.85 | 2.64E−05 | ||||||
hsa‐miR‐30d‐3p | −0.61 | 5.87E−03 | ||||||
hsa‐miR‐32‐3p | −0.75 | 8.85E−05 | ||||||
hsa‐miR‐324‐5p | −0.62 | 1.07E−03 | ||||||
hsa‐miR‐328‐3p | −0.77 | 1.29E−03 | ||||||
hsa‐miR‐331‐3p | −0.74 | 1.09E−03 | ||||||
hsa‐miR‐335‐3p | −0.82 | 4.57E−04 | ||||||
hsa‐miR‐337‐3p | X | −0.71 | 1.83E−02 | −0.83 | 1.91E−02 | |||
hsa‐miR‐337‐5p | X | −0.69 | 2.41E−02 | −0.87 | 4.76E−03 | |||
hsa‐miR‐338‐3p | −0.80 | 5.37E−04 | −0.86 | 4.24E−02 | ||||
hsa‐miR‐33a‐3p | −0.67 | 2.14E−02 | ||||||
hsa‐miR‐33a‐5p | −0.67 | 1.52E−02 | ||||||
hsa‐miR‐340‐5p | −0.75 | 1.07E−04 | ||||||
hsa‐miR‐3611‐3p | −0.73 | 1.13E−03 | ||||||
hsa‐miR‐3617‐5p | −0.85 | 1.32E−04 | ||||||
hsa‐miR‐365a‐3p | 0.60 | 6.57E−03 | ||||||
hsa‐miR‐3667‐5p | −0.67 | 2.29E−02 | ||||||
hsa‐miR‐369‐3p | X | −0.68 | 1.90E−02 | −0.96 | 7.59E−03 | |||
hsa‐miR‐369‐5p | X | −0.63 | 2.48E−02 | −0.90 | 2.78E−02 | |||
hsa‐miR‐370‐3p | −0.81 | 8.12E−03 | ||||||
hsa‐miR‐374a‐5p | −0.93 | 2.39E−06 | ||||||
hsa‐miR‐374b‐3p | −0.79 | 9.37E−05 | ||||||
hsa‐miR‐374b‐5p | −0.79 | 4.16E−05 | ||||||
hsa‐miR‐376a‐1‐3p | X | −0.70 | 8.44E−03 | |||||
hsa‐miR‐376a‐1‐5p | X | −0.90 | 2.51E−04 | |||||
hsa‐miR‐376b‐3p | X | −1.01 | 1.19E−03 | −0.71 | 2.75E−02 | |||
hsa‐miR‐376c‐3p | X | −0.66 | 2.17E−02 | −0.80 | 1.85E−02 | |||
hsa‐miR‐376c‐5p | X | −1.32 | 1.58E−03 | |||||
hsa‐miR‐377‐3p | X | −0.82 | 1.26E−02 | −0.89 | 5.77E−03 | −1.19 | 1.46E−02 | |
hsa‐miR‐378c‐5p | 0.70 | 8.26E−04 | ||||||
hsa‐miR‐379‐3p | X | −0.80 | 4.61E−03 | −0.83 | 4.30E−02 | |||
hsa‐miR‐379‐5p | X | −0.73 | 2.04E−02 | −0.82 | 3.12E−02 | |||
hsa‐miR‐380‐3p | X | −0.66 | 1.45E−02 | −1.08 | 3.48E−03 | |||
hsa‐miR‐381‐3p | X | −0.72 | 9.41E−03 | |||||
hsa‐miR‐382‐3p | X | −0.80 | 3.11E−02 | |||||
hsa‐miR‐409‐5p | X | −0.81 | 2.53E−03 | |||||
hsa‐miR‐410‐3p | X | −0.77 | 1.54E−02 | |||||
hsa‐miR‐411‐3p | X | −0.72 | 1.34E−02 | |||||
hsa‐miR‐411‐5p | X | −0.64 | 1.86E−02 | −0.84 | 3.90E−02 | |||
hsa‐miR‐421‐5p | −0.61 | 3.33E−03 | ||||||
hsa‐miR‐431‐3p | X | −0.63 | 1.04E−02 | |||||
hsa‐miR‐431‐5p | X | −0.67 | 3.01E−02 | −0.70 | 4.89E−02 | |||
hsa‐miR‐432‐3p | X | −0.68 | 4.51E−03 | |||||
hsa‐miR‐4326‐5p | 0.66 | 1.01E−02 | ||||||
hsa‐miR‐450a‐1‐5p | −0.61 | 4.93E−03 | ||||||
hsa‐miR‐4532‐5p | 0.84 | 4.07E−02 | ||||||
hsa‐miR‐454‐3p | −0.64 | 9.36E−05 | ||||||
hsa‐miR‐4732‐5p | 0.76 | 1.15E−05 | ||||||
hsa‐miR‐483‐5p | 1.18 | 5.55E−04 | 1.33 | 1.08E−02 | ||||
hsa‐miR‐485‐3p | X | −0.66 | 1.90E−02 | |||||
hsa‐miR‐486‐1‐5p | 0.75 | 9.07E−06 | 0.63 | 3.05E−02 | ||||
hsa‐miR‐487a‐3p | X | −0.77 | 6.01E−03 | |||||
hsa‐miR‐487b‐3p | X | −0.68 | 1.38E−02 | −0.67 | 4.82E−02 | |||
hsa‐miR‐487b‐5p | X | −0.83 | 2.40E−02 | |||||
hsa‐miR‐491‐5p | −0.74 | 8.20E−04 | ||||||
hsa‐miR‐493‐3p | X | −0.74 | 1.01E−02 | |||||
hsa‐miR‐493‐5p | X | −0.72 | 1.08E−02 | |||||
hsa‐miR‐494‐3p | X | −0.76 | 7.83E−03 | |||||
hsa‐miR‐495‐3p | X | −0.75 | 8.20E−03 | −0.67 | 4.24E−02 | |||
hsa‐miR‐495‐5p | X | −0.64 | 9.50E−03 | |||||
hsa‐miR‐505‐5p | 0.61 | 1.36E−02 | ||||||
hsa‐miR‐512‐1‐5p | X | −0.85 | 4.14E−02 | |||||
hsa‐miR‐515‐1‐3p | X | −0.82 | 2.35E−02 | |||||
hsa‐miR‐515‐1‐5p | X | −0.97 | 2.45E−02 | |||||
hsa‐miR‐516b‐1‐5p | X | −0.91 | 3.62E−02 | |||||
hsa‐miR‐517a‐3p | X | −0.96 | 1.90E−02 | |||||
hsa‐miR‐517c‐3p | X | −1.04 | 1.92E−02 | |||||
hsa‐miR‐518b‐3p | X | −1.19 | 1.50E−02 | |||||
hsa‐miR‐518c‐3p | X | −1.03 | 4.42E−02 | |||||
hsa‐miR‐518f‐3p | X | −0.94 | 2.25E−02 | |||||
hsa‐miR‐518f‐5p | X | 1.39 | 1.15E−03 | |||||
hsa‐miR‐5193‐3p | −0.64 | 2.46E−03 | ||||||
hsa‐miR‐519d‐3p | X | −0.95 | 3.93E−02 | |||||
hsa‐miR‐520a‐3p | X | 0.66 | 1.61E−02 | |||||
hsa‐miR‐520d‐5p | X | −1.01 | 8.36E−03 | |||||
hsa‐miR‐524‐5p | X | −0.94 | 4.56E−02 | |||||
hsa‐miR‐525‐5p | X | −1.26 | 1.22E−02 | |||||
hsa‐miR‐526b‐5p | X | −0.79 | 4.88E−02 | |||||
hsa‐miR‐539‐3p | X | −0.84 | 3.66E−03 | −0.92 | 1.22E−02 | |||
hsa‐miR‐545‐5p | −0.79 | 2.36E−05 | ||||||
hsa‐miR‐548a‐1‐3p | −0.76 | 8.36E−03 | ||||||
hsa‐miR‐548a‐3‐5p | −0.71 | 2.35E−03 | ||||||
hsa‐miR‐548ax‐3p | −0.68 | 1.47E−03 | ||||||
hsa‐miR‐548ax‐5p | −0.79 | 7.02E−04 | ||||||
hsa‐miR‐551b‐3p | −0.71 | 2.24E−03 | −0.74 | 1.45E−02 | ||||
hsa‐miR‐556‐3p | −0.73 | 6.56E−04 | ||||||
hsa‐miR‐556‐5p | −0.85 | 4.70E−04 | ||||||
hsa‐miR‐582‐5p | 0.61 | 2.23E−02 | ||||||
hsa‐miR‐589‐3p | 0.71 | 2.26E−03 | ||||||
hsa‐miR‐590‐3p | −1.25 | 6.34E−07 | −0.68 | 2.98E−02 | ||||
hsa‐miR‐590‐5p | −0.76 | 4.93E−05 | ||||||
hsa‐miR‐625‐5p | −0.61 | 2.70E−03 | ||||||
hsa‐miR‐627‐3p | −0.63 | 3.78E−03 | ||||||
hsa‐miR‐628‐5p | −0.75 | 2.27E−06 | ||||||
hsa‐miR‐6516‐5p | −0.67 | 1.47E−03 | ||||||
hsa‐miR‐654‐3p | X | −0.63 | 3.71E−02 | |||||
hsa‐miR‐655‐3p | X | −0.69 | 1.30E−02 | −0.91 | 8.58E−03 | |||
hsa‐miR‐656‐3p | X | −1.01 | 4.12E−03 | |||||
hsa‐miR‐664a‐3p | −0.66 | 5.82E−05 | ||||||
hsa‐miR‐671‐5p | −0.64 | 2.55E−03 | 0.74 | 1.23E−02 | ||||
hsa‐miR‐6741‐3p | −0.62 | 1.52E−03 | ||||||
hsa‐miR‐744‐3p | −0.65 | 1.24E−03 | ||||||
hsa‐miR‐758‐3p | X | −0.91 | 4.00E−03 | |||||
hsa‐mir‐7641‐2 | 0.65 | 6.42E−03 | ||||||
hsa‐miR‐877‐3p | −0.62 | 1.14E−02 | ||||||
hsa‐miR‐889‐3p | X | −0.60 | 2.98E−02 | −0.68 | 3.14E−02 | |||
hsa‐miR‐92a‐2‐3p | 0.73 | 9.10E−03 | ||||||
hsa‐miR‐99b‐3p | −0.60 | 1.25E−02 |
Of the 668 detectable miRNAs in whole plasma (631 shared plus 20 in control and 17 in PTL), 132 showed a significant concentration difference (≥1.5 change and P‐value ≤.05) between the preterm and normal gestation groups. Likewise, 51 of the 535 detectable miRNAs in EVs (476 shared plus 36 in control and 23 in PTL) had significant concentration changes between the preterm and normal gestation groups. Only 10 of the 585 detectable miRNA in the EV‐depleted plasma samples (486 shared plus 98 in control and 1 in PTL) had significant concentration changes between the two groups.