Fig 7. Rested effector T cells protect from parasitemia and pathology.
A) Schematic of the experimental design. Effector T cell subsets were sorted from the spleens of B5 TCR Tg at d8 p.i. and transferred (5 x 105) into RAG2o mice for 60 days before infection. Recipients were then infected with 105 P. chabaudi. Parasitemia, weight, and temperature change was measured for 14 days after infection. B) Average peak parasitemia (%iRBC/RBC) of each mouse (d8-10 p.i.) is shown. As measurements of pathology, the average of C) weight and D) temperature loss (% change) of each recipient at the peak of pathology (d8-10 p.i.) are shown. Flow cytometry was performed to quantitate E) the number of B5 T cells (CD4+Thy1.2+) recovered from RAG2o recipients on day 14 p.i. and F) graph showing MFI of IFN-γ in T cells recovered from each group. Data represent 2–3 mice per group and are representative of 3 similar independent experiments. Parasitemia was analyzed using one-way ANOVA and Tukey’s post-hoc. Error bar represents SEM, * p<0.05, ** p<0.01, *** p<0.001, n.s—not significant.