Fig 3. Induction of certain TLOs altered flocculation and cell aggregation.

A. Tet-regulated TLO strains were grown overnight in the presence of 50 μg/ml Dox. Vortexed cultures were set to an OD₆₀₀ of 2.0 in a channel cuvette and allowed to settle. Absorbance readings were taken every 15 minutes to measure the rate of flocculation (cell settling). Each data line represents six biological replicates. B. Relative fold change of flocculation for +Dox/-Dox is plotted for the 150-minute timepoint where change is normalized relative to SC5314. Error bars indicate standard deviations. C. Strains grown overnight in the presence or absence of 50 μg/ml Dox were diluted 1:2 and visualized by light microscopy. D. The number of cells per aggregate was quantified for all cells across 10 random fields of view per strain and plotted with standard error bars. A legend indicates the representative TLO gene for each color where solid bars indicate +Dox and hatched bars indicate -Dox. * denotes p < 0.05.