Exponentially growing cells of each MB line were treated with increasing concentrations of Vis (1-100 μM), BEZ (1-100 nM) and Cis (0.1-10 μM) or DMSO (vehicle, 0.05%)) in 96-well plates and the growth of these cells was determined at 72 hours using MTT assay. The percentage of cell viability is relative to control vehicle-treated cells. The values represent the means ± SD from four wells of 96-well plates. (A-D) Show the dose-response effects of inhibitors Vis and BEZ and (E) shows the dose-response effect of Cis in all four MB lines as indicated. (F) IC50 values of the indicated inhibitors and Cis in four MB cell lines. Note: The agents Vismodegib, BEZ235 and cisplatin are abbreviated as Vis, BEZ and Cis, respectively, throughout all Figures.