Figure 1. Single agent effects of Vismodegib (Vis), BEZ235 (BEZ) and cisplatin (Cis) on MB cell growth in vitro.

Exponentially growing cells of each MB line were treated with increasing concentrations of Vis (1-100 μM), BEZ (1-100 nM) and Cis (0.1-10 μM) or DMSO (vehicle, 0.05%)) in 96-well plates and the growth of these cells was determined at 72 hours using MTT assay. The percentage of cell viability is relative to control vehicle-treated cells. The values represent the means ± SD from four wells of 96-well plates. (A-D) Show the dose-response effects of inhibitors Vis and BEZ and (E) shows the dose-response effect of Cis in all four MB lines as indicated. (F) IC₅₀ values of the indicated inhibitors and Cis in four MB cell lines. Note: The agents Vismodegib, BEZ235 and cisplatin are abbreviated as Vis, BEZ and Cis, respectively, throughout all Figures.