Figure 3. Combination efficacy of Vis and BEZ on associated pathways/molecules.
(A) Daoy and HD-MB03 MB cells were treated with 20 μM Vis and 20 nM BEZ alone or combined for 24 hours and the expression of PI3K-mTOR and HH pathways associated key molecules were determined using western blot analyses. β-Actin was used as a loading control in these experiments. (B) Bar graphs show the quantification of protein expression relative to the control (DMSO) in Daoy and HD-MB03 MB cells after β-Actin normalization using the Image-J software. *p<0.05; **p<0.01.