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. 2018 Mar 30;9(24):16619–16633. doi: 10.18632/oncotarget.24618

Figure 5. Effects of inhibitors in combination with chemotherapy on MB apoptosis and colony formation.

Figure 5

(A) Shows a representative scatter diagram for the apoptotic cell analyses following treatment with vehicle (DMSO) or Vis 20 μM, BEZ 20 nM and Cis 1 μM alone or combined as indicated in MB cells. (B) Quantification of the apoptotic cells (% Annexin-V/PI) following inhibitors alone or in combination with cisplatin treatment in D-283 and HD-MB03 MB cells. The values represent the means ± SD of three separate experiments. *, p<0.05; **, p<0.01. (C) Shows a representative micrograph for the colony forming ability of MB cells with the treatments of vehicle or Vis (20 μM), BEZ (20 nM) and Cis (1 μM) alone or combined for 15 days. The micrographs were taken using a phase contrast microscope at 10x magnification. Scale bar; 100 μm. (D) Quantification of the number of colonies following treatment with inhibitors alone or combined in Daoy, D-283 and HD-MB03 MB cells. The values represent the means ± SD from three wells of 6-well plates. *p<0.01. (E) Western blot analyses for the expression levels of CD133 and SOX2 following the indicated treatments of HD-MB03 spheres for 48 hours. β-Actin was used as a loading control in this experiment.